TABLE 7.

Prediction of potential immunogenicity by in silico analysis for parent and hetero-IgG1 antibodies

The TEPITOPE algorithm was utilized to identify potential non-tolerant agretopes which could bind to HLA class II molecules and elicit immune responses. Tertiary structural information of the antibodies was not considered in this analysis. The linear 9 residue peptides that could bind to HLA class II molecules are listed. The 8 DRB1 alleles cover 95% of human populations. The red colored “D” and “K” indicate the engineered residues.