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. 2015 Jan 28;290(12):7563–7575. doi: 10.1074/jbc.M114.609487

FIGURE 5.

FIGURE 5.

Regulation of gene transcription by Foxf2. A, increased expression of Foxf1, Wnt, bone morphogenetic protein, as well as members of the PDGF, Hedgehog, and SRF pathways in smooth muscle isolated from adult Tg(smMHC-Cre-eGFP+/−);Foxf2−/− colons as determined by qRT-PCR. B, Indian Hedgehog (IHH) expression was increased in whole colon RNA from adult Tg(smMHC-Cre-eGFP+/−);Foxf2−/− mice. Epidermal growth factor receptor (EGFR) expression was unaltered. Expression levels were normalized to β-actin (n = 4). *, p < 0.05 versus control. C, FOXF1-positive myocytes were more abundant in Tg(smMHC-Cre-eGFP+/−);Foxf2−/− smooth muscle than control myocytes as shown by immunostaining of the adult colon. Despite prolonged proliferation and altered gene expression, muscle markers αSMA and γSMA were observed in Tg(smMHC-Cre-eGFP+/−);Foxf2−/− colon. Fibrosis was not observed in adult Tg(smMHC-Cre-eGFP+/−);Foxf2−/− colons as determined by Masson's Trichrome staining of intestinal paraffin sections. D, vasculature was unaltered in adult Tg(smMHC-Cre-eGFP+/−);Foxf2−/− colons as visualized by PECAM-1 staining. Epithelial staining for FOXA2 and cytokeratin was normal in adult colons. Scale bar, 10 μm.

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