FIGURE 4.

Inhibitory effect of C4-modified xylosides on hβ4GalT7 activity. A, chemical structures of the xyloside analogs synthesized and tested as inhibitors. From left to right: 4H-Xyl-NP, 4H-Xyl-MU, and 4F-Xyl-MU. B, inhibition assays using purified recombinant wild-type hβ4GalT7 in the presence of fixed 4-MUX (0.5 mm) and UDP-Gal (1 mm). Activities are presented as function of the logarithm of increasing inhibitor concentrations (0–5 mm); 4H-Xyl-NP (▴), 4H-Xyl-MU (■), and 4F-Xyl-MU (○). Results are the mean ± S.E. of three independent determinations on assays performed in duplicate.