FIGURE 3.

Binding affinities and potencies of ACTH(1–24) analogs at WT hMC2R. Cells transfected with hMC2R were incubated with ¹²⁵I-ACTH(1–24) at 37 °C for 1 h in the presence of the indicated amounts of unlabeled ligands. Total ¹²⁵I-ACTH(1–24) binding was then determined on duplicate wells as described under “Experimental Procedures.” Data points represent the mean ± S.E. of at least three independent experiments. A depicts the binding affinities of ACTH(1–24) analogs at WT hMC2R. B demonstrates the abilities of ACTH(1–24) analogs to stimulate the production of intracellular cAMP at WT hMC2R.