Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 27;290(12):7722–7735. doi: 10.1074/jbc.M114.628008

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Established fluorescence methods are reliable for measuring G4/duplex DNA unwinding. A, Pif1-mediated G4-DNA/duplex DNA (substrate S26G4D17) unwinding in the absence and in the presence of 5 μm TMPyP4. B, no inhibitory effect of TMPyP4 and BG4 on the duplex DNA (S26D17) unwinding by Pif1. C, influence of the concentration of TMPyP4 on Pif1-mediated G4/duplex DNA unwinding. D, influence of the concentration of the G4 antibody (BG4) on Pif1-mediated G4/duplex DNA unwinding. The above kinetics were performed with 80 nm Pif1 and 2 nm fluorescence-labeled DNA substrates under experimental conditions as indicated under “Experimental Procedures.”