FIGURE 2.

Growth curves of the P. aeruginosa WT and deletion strains supplemented with heme or iron. A, M9 media no iron. PAO1 wild type (open circles), ΔphuR (closed circles), ΔhasR (open squares), and ΔphuR::gmΔhasR::tc (closed squares). B, as in A supplemented with 100 μm FeCl₃. C, supplemented with 0.5 μm heme. D, supplemented with 5 μm heme. Growth curves were generated using a Bioscreen C MBR (Growth Curves USA) with continuous shaking at 37 °C and the A₆₀₀ was recorded after every 30 min over a 24-h time period. Values represent the standard deviation of three separate experiments.