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Expression of the heme utilization proteins in the PAO1 WT, ΔphuR, and ΔhasR strains. A, Western blot analysis of PhuR (upper panel) and HasR (lower panel) at the 8-h time point in P. aeruginosa strains as indicated. B, Western blot analysis of PhuS (upper panel) and HemO (lower panel) at the 8-h time point in P. aeruginosa strains as described under “Experimental Procedures.”
