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BVIX isomer fragmentation patterns for PAO1 WT and mutant strains supplemented with 5 μm heme. A, LC-MS/MS fragmentation pattern of the eluting isomers in PAO1 wild type. B, as in A for the ΔhasR strain. C, as in A for the ΔphuR strain. D, as in A for the ΔphuR::gmΔhasR::tc strain. LC-MS/MS was performed as described under “Experimental Procedures” with multiple reaction monitoring.
