TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | F′, araD (lac-proAB) rpsL ϕ80dlacZ DM15 hasd R17 | Stratagene |
| S17-1 | pro thi hsdR_ Tpr Smr; chromosome::RP4–2 Tc::Mu-Km::Tn7/λpir | 50 |
| P. aeruginosa | ||
| PAO1 | Wild type | |
| PAO1 ΔphuR::gmFRT | PAO1 with 0.4 kb of phuR replaced by GmR cassette harboring flanking FLP recombinase sites | Oglesby-Sherrouse |
| PAO1 ΔhasR::tcFRT | PAO1 with 1.5 kb of hasR replaced by TcR cassette harboring flanking FLP recombinase sites | Oglesby-Sherrouse |
| PAO1 ΔphuR::gmΔhasR::tc | PAO1 with 0.4 kb of phuR replaced by GmR and 1.5kb of hasR replaced by TcR cassettes harboring flanking FLP recombinase sites | Oglesby-Sherrouse |
| PAO1 ΔphuR | PAO1 with 1.1 kb of phuR gene removed by flip recombinase | This study |
| PAO1 ΔhasR | PAO1 with 1.5 kb of hasR gene removed by flip recombinase | This study |
| Plasmids | ||
| pFLP2 | AmpR; source of FLP recombinase | 51 |
| pMRL2 | AmpR; pET-11a derivate harboring a water-soluble domain of the rat liver cytochrome b5 | 41 |