TABLE 1.

List summarizing the yeast strains used in this study

The abbreviations used are as follows: ΔPUT2, knockout strain for the proline utilization 2 gene (PUT2); control, control strain; TcP5CDH, complemented strain harboring cds for P5CDH in T. cruzi. The WT strain lacks auxotrophy, whereas the ΔPUT2 strain exhibits auxotrophy for HIS, MET, LEU, and URA and is also deficient in P5CDH activity. Both control and TcP5CDH strains exhibit auxotrophy only for MET, whereas the control strain is unable to grow when proline is the only nitrogen source.

Strain	Name	Plasmids inserted	Resultant genotype
S288c	WT	None	MATα; SUC2; GAL2; MAL; MEL; FLO1; FLO8-1; HAP1; HO; BIO1; BIO6
BY4741	ΔPUT2	None	MATa; HIS3Δ1; LEU2Δ0; MET15Δ0; URA3Δ0; YHR037w::KANMX4
	Control	pYEp351, pYES-HIS3	MATa; MET15Δ0; YHR037w::KANMX4
	TcP5CDH	pYEp351, pYES-HIS3, pYES-TcP5CDH	MATa; MET15Δ0; YHR037w::KANMX4; TcP5CDH