FIGURE 10.

Homology modeling of fibrillar medin. A, model of medin fibrils created in Modeler using the Aβ(1–40) structural model (Protein Data Bank entry 2LMN) as a template and the sequence alignment in Fig. 1B. No further refinement of the model was performed. The left view (perpendicular to the fibril axis) shows the turn region stabilized by the salt bridge between Asp²⁵ (red) and Lys³⁰ (blue). The right view (down the fibril axis) shows Trp¹¹ and Trp²¹ (orange) and Ile³⁵ and Ile³⁶ (cyan), with close packing of Trp²¹ and Ile³⁵. B, simulated DARR spectrum (red) calculated from ¹³C chemical shifts predicted from the structural model using SHIFTX2, superimposed on the experimental spectrum (black).