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D25N forms non-toxic prefibrillar oligomers. A, immunoblot analysis of WT and D25N aggregates using A11 and OC conformer-specific antibodies. B, immunoblot analysis of D25N aggregates using A11 conformer-specific antibodies, including a stable Aβ oligomer (45) positive control and a BSA negative control. C, Cell Counting Kit-8 cell viability assay performed on human aortic smooth muscle cells. *, statistical significance (p < 0.01). Error bars, S.D.
