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Comparison of WT and mutant kinetic parameters measured by ThT analysis
S.E. values for fits are given in parentheses. RFU, relative fluorescence units.
| WT | D25N | |
|---|---|---|
| Maximum fluorescence intensity | 716.07 (1.92) | 567.31 (2.56) |
| Rate (h−1) | 1.28 (0.04) | 1.43 (0.08) |
| R2 | 0.985 | 0.951 |
