TABLE 3.
Parameters associated with fluorescence anisotropy decays
The time-resolved fluorescence anisotropy decays of all low molecular weight (LMW) samples fitted satisfactorily to a two-exponential function (Equations 4–6). The rotational correlation times (φ) and their amplitudes (β) derived from the fitting are shown. The error associated with rotational correlation time and its amplitude is ∼10–20%.
| LMW sample | φ1 (ns) (β1) | φ2 (ns) (β2) |
|---|---|---|
| [V3W]WT | 0.36 (0.67) | 4.5 (0.33) |
| [V71W]WT | 0.25 (0.56) | 3.12 (0.44) |
| [A124W]WT | 0.21 (0.45) | 2.48 (0.55) |
| [A140W]WT | 0.13 (0.72) | 1.70 (0.28) |
| [V3W]E46K | 1.3 (0.1) | 6.1 (0.9) |
| [V71W]E46K | 0.31 (0.66) | 3.80 (0.34) |
| [A124W]E46K | 1.3 (0.1) | 6.1 (0.9) |
| [A140W]E46K | 0.57 (0.60) | >10 (0.40) |
| [V3W]A53T | 0.36 (0.64) | 5.4 (0.36) |
| [V71W]A53T | 0.29 (0.60) | 3.70 (0.40) |
| [A124W]A53T | 0.5 (0.46) | 6.83 (0.54) |
| [A140W]A53T | 0.20 (0.66) | 3.36 (0.34) |
| [V3W]A30P | 0.36 (0.65) | 4.5 (0.35) |
| [V71W]A30P | 0.27 (0.58) | 3.70 (0.42) |
| [A124W]A30P | 0.35 (0.57) | 3.2 (0.43) |
| [A140W]A30P | 0.35 (0.70) | 4.60 (0.30) |