FIGURE 6.

GX sPLA₂ processing by furin or PCSK6 enhances GX sPLA₂-dependent inhibition of LXR-mediated gene activation. HEK 293 cells were transiently co-transfected with ptk-3× LXRE-luc reporter construct and vectors encoding mLXRα, mRXR, Renilla luciferase, and either a control vector encoding GFP (293-C) or a vector encoding 3× FLAG-tagged GX sPLA₂ in the absence (293-GX) or presence of furin (293-GX+F) (A) or PCSK6 (293-GX+P) (B). Cells were then incubated in media containing either 0 or 1 μm T0901317 for 24 h prior to measurements of luciferase activity. Data are means ± S.E. (n = 4) and are representative of two independent experiments. *, p < 0.05; ***, p < 0.001; NS, not significant.