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. 2015 Jan 26;290(12):7871–7886. doi: 10.1074/jbc.M114.605386

FIGURE 3.

FIGURE 3.

Abcb6 deficiency attenuates constitutive expression of hepatic P450s. a, Cyp3a11, Cyp2b10, and Cyp1a2 but not Cyp2e1 mRNA expression was down-regulated in Abcb6−/− mice. mRNA expression was determined by real time RT-PCR. The data are presented as means ± S.E. from four independent measurements with three mice per group per experiment. *, significantly different from Abcb6+/+ mice; p < 0.01. #, significantly different from Abcb6+/− mice; p < 0.01. b, left panel, expression of hepatic Cyp3a11, Cyp2b10, and Cyp1a2 but not Cyp2e1 or P450 oxidoreductase (POR) protein is attenuated in Abcb6−/− mice. Protein expression was determined by immunostaining using protein specific antibodies as described under “Experimental Procedures.” The data are representative of three independent experiments with three mice per group per experiment. Right panel, cumulative (average of all mice in the three independent experiments) ImageJ analysis of Cyp3a11, Cyp2b10, Cyp1a2, and Cyp2e1 immunostained protein bands in Abcb6+/+ and Abcb6−/− mice liver. The data are normalized to Gapdh and presented as means ± S.E. *, significantly different from Abcb6+/+ mice; p < 0.01. c, microarray analysis of hepatic P450 mRNA expression in Abcb6−/− mice shows a pattern that is similar to the one observed using real time RT-PCR analysis. d–f, real time RT-PCR analysis of hepatic P450 mRNA in Abcb6 deficiency shows Abcb6 allele-specific (d), non-allele-specific (e), and Abcb6-independent (f) expression pattern. The data are presented as means ± S.E. from three independent measurements with three mice per group per experiment. *, significantly different from Abcb6+/+ mice; p < 0.01. #, significantly different from Abcb6+/− mice; p < 0.01.