TABLE 1.
Plasmids used in this study
| Plasmid | Description | References |
|---|---|---|
| pKC1139 | Temperature-sensitive vector for gene knock-out | (22) |
| pKC1139-atrA | atrA in-frame deletion plasmid | This study |
| pKC1139-adpA | adpA in-frame deletion plasmid | This study |
| pKC1139-arpA | arpA in-frame deletion plasmid | This study |
| pSET152 | Integrative plasmid for complementation in Streptomyces | (22) |
| pSET152-atrA | atrA gene complementation plasmid | This study |
| pSET152-adpA | adpA gene complementation plasmid | This study |
| pSET152-arpA | arpA gene complementation plasmid | This study |
| pTA2 | T vector | Toyobo Co. |
| pTA2-dptEp | dptE promoter (dptEp) cloned in pTA2 vector | This study |
| pTA2-atrAp | atrA promoter (atrAp) cloned in pTA2 vector | This study |
| pUC18 | Cloning vector | Novagen |
| pUC18-dptEp | dptEp cloned in BamHI site of pUC18 vector | This study |
| pET32a | E. coli expression vector | Novagen |
| pET32a-atrA | atrA ORF cloned in BamHI/HindIII site of pET32a | This study |
| pET32a-adpA | adpA ORF cloned in BamHI/HindIII site of pET32a | This study |
| pIPP1 | Promoter probing vector with xylE reporter | (23) |
| pIPP1-dptEp | dptEp cloned in BamHI site of pIPP1 | This study |
| pIPP1-atrAp | atrAp cloned in BamHI site of pIPP1 | This study |