Table 2.
Fluorescence applications for OP detection.
| Bio-Receptor | Detection Mechanism | Limitations | Sensitivity |
|---|---|---|---|
| Acetylcholinesterase (AChE) | pH-sensitive fluorescent dye detecting the change in pH after substrate hydrolysis. | Indirect measurement | nM |
| False positives | |||
| Slow response time (minutes) | |||
| Short lifetime due to enzyme degradation. | |||
|
| |||
| Organophosphorus hydrolase (OPH) | pH-sensitive fluorescent dye detecting the change in pH after OP hydrolysis. | Indirect measurement | μM |
| Slow response time | |||
| False positives | |||
| Short lifetime due to enzyme degradation. | |||
|
| |||
| Monoclonal Antibody | Fluorescence Polarization Immunoassay after formation of antigen-antibody complex. | Indirect measurement | mM |
| Very low sensibility | |||
| Requires baseline testing | |||
|
| |||
| Organophosphorus hydrolase (OPH) | Changes in FRET of the Coumarin 1 after OP hydrolysis. | Indirect measurement | μM |