Figure 6. CDSiL-MS based strategy in measuring functional residue-specific conformational rearrangements in β₂AR.

Time-course curves for the extent of NEM reactivity at C327 (a) and SA reactivity at K263 (d) expressed as percent intensity ratio plotted vs. labeling time (%R (t)) on a logarithmic scale, for unliganded (black circles), iso (red squares), ICI (green triangles) or carv (blue diamond). (b, e) Same labeling curves expressed in the form of percent of sites labeled, %F (t) following normalization of %R (t). Bar graphs summarizing the effects of each ligand on the changes in the L-factors of the C327 (c) and K263 (f) on the β₂AR, expressed relative to the unliganded receptor. Inset indicates position of labeled residue in the β₂AR snake like diagram. Data correspond to the means ± standard errors from at least three independent experiments. Asterisk indicates statistical significance (*p < 0.05) compared to control receptor alone by one-way ANOVA.