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• TIMING
| Protein sample preparation and protein-radio-ligand binding assay | Step 1-2 | 1 day |
| Differential NEM and SA labeling reactions | Step 6-7 | 5-6 hours |
| CHCb/MeOH precipitation | Step 8-16 | 3-5 hours |
| Protein solubilization, reduction and alkylation | Step 17-19 | 3-4 hours |
| In-solution protein digestion | Step 20-23 | 16 hours |
| Peptide desalting | Step 24 | 4-5 hours |
| MALDI-TOF/TOF-MS and data analysis | Step 25-34 | 4-6 hours |
| Quantitation of site-specific labeling kinetics | Step 35-61 | 1 day |
