FIG. 1.

Effect of JQ1 on embryonic stem cells (ESCs). (A) Phase contrast micrograph of ESCs grown in LIF-containing medium exposure to 1 μM JQ1 for 72 h showing morphology (upper panel) and alkaline phosphatase staining (middle panel) and immunolabeling of SSEA1 (lower panel); (B) BRD4 immunolabeling of control and JQ1-treated ESCs with DAPI counterstain (upper panel) and Nanog immunolabeling showing JQ1-induced downregulation (lower panel). Color images available online at www.liebertpub.com/scd