FIG. 4.

Overexpression of Nanog attenuates JQ1-induced downregulation of Nanog. (A) Photomicrographs showing morphology (above) and alkaline phosphatase staining (below) of ESCs transfected with pNanog (CAG vector) or pControl (empty vector) and treated with JQ1. (B) Nanog expression levels of stably transfected ESCs showing effect of JQ1 at 1 μM for 24 h as determined by qRT-PCR. (LIF- vs. LIF+ vs. LIF+/JQ1 for pNanog and pControl ESCs). Data are representative of at least three independent experiments performed in triplicate±SD. Color images available online at www.liebertpub.com/scd