FIG. 5.

Brd4 knockdown induces ESC differentiation and downregulation of Nanog. (A) Phase contrast micrographs of ESCs transfected with scramble siRNA control versus Brd4-specific siRNA. (B) Graph showing level of Brd4 knockdown and effect on Nanog expression compared with siRNA control as determined by qRT-PCR. (C) Chromatin immunoprecipitation of BRD4 at the promoter regions of Nanog and GAPDH in ESC following 24 h growth in the presence of 1 μM JQ1 or (−)-JQ1, or following mock manipulation with ethanol. Enrichment is shown as a percentage of input, and in comparison to IgG enrichment. Data are representative of two independent experiments performed in triplicate±SD.