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. 2015 Mar 11;4(4):333–338. doi: 10.5966/sctm.2013-0172

Figure 1.

Figure 1.

Establishment of FA-iPSC lines from patients with FA and mutations in FANCA. CiRA00115 is a control iPSC line. (A): The experimental scheme. (B): The morphology and immunostaining of pluripotent markers of FA-iPSCs and teratoma formation by the FA-iPSC lines (ectoderm: neural rosette; mesoderm: cartilage; endoderm: gut-like structure). Scale bars indicate 100 μm. (C): The results of karyotype analysis of fibroblasts and iPSCs from FA patients. The karyotypes of the fibroblasts were 46XX and 46XY for FA02 and FA07, respectively. The karyotype of FA-iPSCs from FA02 was summarized as 46, XX, t(12;18)(p11.2;p11.2) [20 cells]. The karyotypes of FA-iPSCs from FA07 were as follows: for FA07-2, 46, XY, add(1)(q32) [14 cells] and 46, XY [6 cells]; for FA07-3, 42-46, XY, add(8)(q24.1),add(11)(q23),del(11)(q23),add(18)(q21) [17 cells] and 42-46, XY, add(8)(q24.1),add(9)(q34),add(21)(p11.2) [3 cells]; for FA07-4, 45-46, XY, add(8)(q24.1),add(9)(q34),add(21)(p11.2) [20 cells]. Twenty metaphases were analyzed for each sample. Abbreviations: cFA, FANCA-complemented FA-iPSC clone; FA, Fanconi anemia; HAPC, hemoangiogenic progenitor cell; iPSC, induced pluripotent stem cell.