Figure 3.

The defective differentiation propensity in Fanconi anemia induced pluripotent stem cell-derived hemoangiogenic progenitors. KhES1 and 409B2 are control embryonic and induced pluripotent stem cell lines, respectively. (A): A schematic diagram of hematopoietic differentiation. (B–E): The number of differentiated cells derived from 5,000 sorted KDR⁺CD34⁺ cells. (B): CD34⁺CD45⁺ hematopoietic progenitors. (C): CD33⁺CD45⁺ myeloid cells. (D): CD45⁻CD235α⁺ erythroid cells. (E): CD31⁺CD34⁺ endothelial cells. All data are presented as mean ± SD and are representative of three independent experiments. All p values were determined by Wilcoxon rank sum test. ∗, p < .05; ∗∗, p < .01; ∗∗∗, p < .001 (n = 3). Abbreviations: α-MEM, alpha Minimum Essential Medium; bFGF, basic fibroblast growth factor; EPO, erythropoietin; IL-3, interleukin 3; NS, not significant; SCF, stem cell factor; TPO, thyroid peroxidase; VEGF, vascular endothelial growth factor.