Figure 1.

The 4−/4+ staged embryoid bodies (EBs) are immunoreactive for neural markers. (A, B): EB cells expressed positive for neural phenotypes, including neuronal (anti-TUJ-1 [A]) and astrocytic (anti-GFAP [B]) expression. (C, D):The majority of EB cells demonstrated early tripotential neural progenitor predominance, positive for oligodendroglial (anti-NG2, red [C]) and neural (anti-nestin, green; anti-NG2, red [D]) precursors. (E, F): However, other stages of oligodendroglial showed differentiation as well (anti-A2B5 [E] and anti-O1 [F]). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 50 µm. Abbreviations: GFAP, glial fibrillary acidic protein; NG2, nerve glial antigen 2.