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. 2015 Feb 23;4(4):309–319. doi: 10.5966/sctm.2014-0183

Figure 1.

Figure 1.

Generation of MKs derived from human induced pluripotent stem cells (hiPSCs). (A): Schematic diagram for MK generation and expression of CD34, CD45, CD41, and CD42a during hiPSC differentiation. (B): Representative flow cytometry dot plots of suspension cells on day 14 (left) and day 19 (right). The suspension cells expressed MK markers CD41 and CD42a and hematopoietic cell markers CD34 and CD45. Ig isotype controls for CD41, CD42a, CD34, and CD45 were used to determine the background. (C): Phase contrast images of suspension cells on days 14 and 19. The yellow arrows indicate some of the morphologically larger cells on day 19. (D): The cell number increment of suspension cells from day 14 to day 19 in one well of a 6-well plate. Mean ± SD; n = 3. Scale bars = 100 μm. Abbreviations: BMP4, bone morphogenetic protein 4; D, day; EB, erythroid body; EGF2, epidermal growth factor 2; FGF-2, fibroblast growth factor 2; HPCs, hematopoietic progenitor cells; IL, interleukin; MK, megakaryocyte; SCF, stem cell factor; TPO, thrombopoietin.