Figure 4.

Replacing BSA with HuA in serum-free medium (SFM) increased the efficiency and consistency of hematopoietic progenitor cell (HPC) differentiation. (A): Representative images of day 2 and day 14 erythroid bodies from failed (left) and successful (right) differentiations using various lots of BSA and HuA preparations in SFM. Scale bars = 100 μm. (B): CD34⁺CD45⁺ HPCs generated from one 96-well plate of BC1 using SFM containing either BSA product or HuA of 5 different lot numbers each. Solid dots represent BSA group, and hollow dots represent HuA group. Mean ± SD; n = 15. Abbreviations: BSA, bovine serum albumin; D, day; HuA, human albumin.