FIG. 2.

Gene transfer in the airways of 3- to 6-day-old ferrets. (A–E) Airways after intratracheal delivery of (A) vehicle or (B–E) helper-dependent Ad5 (i.e., gutted adenovirus) with X-Gal staining 8 days postinfection. This vector expresses nt-LacZ under the control of the K18 promoter. (A–D) are sections and (E) is a whole-mount preparation. The transgene is expressed predominantly in the small airways. (F–J) Airways after intratracheal delivery of (F) vehicle or (G–J) equine infectious anemia virus (EIAV) pseudotyped with influenza A virus subtype H7 hemagglutinin (HA) with X-Gal staining 8 days postinfection. This EIAV vector expresses nt-LacZ under the control of the CBA promoter. (F–H) are sections and (I and J) are whole-mount preparations. The transgene is expressed in the large and small airways as well as in alveolar regions. (K–N) Newborn ferrets were infected with an RSVmCherry-encoding FIV-GP64 virus. mCherry expression (red) was observed almost exclusively in bronchioles (Br) at 7 days postinfection. (L) shows only the mCherry channel of the boxed region in (K). (M) is the bright-field image of the fluorescence panel in (N). (K) and (N) are counterstained with DAPI to mark nuclei.