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. 2015 Mar 20;9:93. doi: 10.3389/fnins.2015.00093

Figure 5.

Figure 5

Short, low-dose AZT exposure at the time of withdrawal is sufficient to perturb inducible neurogenesis. At the initiation of serum and mitogen withdrawal MASCs were treated with a single pulse of 0.03, 0.3, or 3 μM AZT for 2, 8, or 24 h (A–C, respectively). All AZT doses suppress neuroblast induction in a dose- and time-dependent manner. Even the lowest concentration of AZT, 0.03 μM, applied for only 2 h at the time of serum and mitogen withdrawal significantly decreases neuroblast formation compared to the control group. In contrast, AZT pre-treatment with the same concentration range for 3 days prior to supplement withdrawal (D) is less disruptive of neurogenesis; there is a dose-related trend in suppressed neuroblast formation, but only the highest dose (3 μM) causes a statistically significant reduction. One-Way ANOVA, Dunnet's Multiple Comparison Test of significance; N = 3 for all groups; *p < 0.05; **p < 0.01. Error bars represent standard deviation.