Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 20;9:93. doi: 10.3389/fnins.2015.00093

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Demir and Laywell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

In utero AZT reduces neurosphere size. AZT was administered to pregnant dams from day 12 of gestation until postnatal day 3 (250 mg/kg/day, s.q.). Single-cell dissociates of SEZ were prepared from pup brains on postnatal day 3 and assayed for neurosphere production. (A) The total number of neurospheres generated from AZT-treated vs.control pups does not differ. (B) While total neuroshere number does not difffer between treated and control groups, In utero AZT causes a clear and significant reduction in the diameter of neurospheres generated from the pups of treated mothers as compared to the pups of untreated control mothers; notice the reduction in the the number of large diameter neurospheres (>120 μM) and a corresponding increase in the number of small and medium diameter neurospheres (40–120 μM) in the the treated group as compared to the controls. Unpaired Student's t-test for (A); One-Way ANOVA for (B) N = 3 for all groups; ^*p < 0.05. Error bars represent standard deviation.