Fig. 3.

Laminin supports thrombus formation under shear. Sodium citrate-anticoagulated whole human blood was co-perfused with a solution of Ca²⁺ /Mg²⁺(7.5 mmol L⁻¹/3.75mmol L⁻¹ final, respectively) over (A) laminin, (B) collagen or (C) BSA surfaces for 12 min at 250 s⁻¹, followed by perfusion of modified Tyrode buffer to remove non-adherent cells. In separate experiments, blood was pretreated with 20 μg mL⁻¹ 1A6, 40 μg mL⁻¹ CTI or 20 μg mL⁻¹ 14E11 for 10 min at 37 °C prior to perfusion. Images were recorded using DIC microscopy prior to lysis and immunoblotting for the fibrin degradation product, D-dimer. Images and blots are representative of at least three experiments.