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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Pharmacol. 2003 May;63(5):1021–1031. doi: 10.1124/mol.63.5.1021

Fig. 4.

Fig. 4

Effect of sodium ions on the equilibrium binding of the agonist radioligand, [125I]I-AB-MECA to WT and mutant ARs. [125I]I-AB-MECA (0.5 nM) was incubated with membranes (8 –20 µg of protein) from COS-7 cells expressing WT or mutant receptors in total assay volume of 200 µl of Tris-HCl buffer, pH 8.0, containing 10 mM MgCl2 and 1 mM EDTA at 25°C for 60 min. Data were from three independent experiments performed in triplicate.