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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Pharmacol. 2003 May;63(5):1021–1031. doi: 10.1124/mol.63.5.1021

TABLE 3.

Dissociation rates of the antagonist radioligand [3H]PSB-11 from WT and mutant receptors in the absence and presence of allosteric modulators

k−1
Control + HMA (100 μM) + Sodium Ions
(100 mM)
WT 0.23 ± 0.02 0.46 ± 0.05* 0.10 ± 0.04*
D58N2.50 0.27 ± 0.04 0.52 ± 0.07* 0.31 ± 0.04
L244A6.49 0.22 ± 0.04 0.45 ± 0.10* 0.11 ± 0.03*
S247A6.52 0.24 ± 0.03 0.47 ± 0.10* 0.12 ± 0.02*
*

Significantly different from control, P < 0.05.

Results were expressed as means ± S.E. from three independent experiments. The dissociation rates (k−1) were determined at 25°C. The final concentration of [3H]PSB-11 used in this experiment was 8 nM.