Figure 5.
IHC staining of D2-40 for LMVD in tumors on pleura from three treatment groups. Positive IHC of D2-40 shows up as a brown stain in each figure. Positive endothelial cells stained by the anti-D2-40 antibody were recognized as lymphatic vessels. The LMVD was counted at ×200. LMVD was significantly reduced in the H-ITCZ-treated group (C) compared with the H-β-C group (A) or the L-ITCZ group (B). Microlymphatic vessels were significantly decreased in the H-ITCZ treated group compared with the H-β-C group or the L-ITCZ group (D). Columns represent the mean value of each group, bars ± SD. ***P<0.001, **P<0.05. IHC, immunohistochemistry; LMVD, lymphatic micro vessel density; H-β-C, hydroxypropyl-β-cyclodextrin; L-ITCZ, low itraconazole; H-ITCZ, high itraconazole.
