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. Author manuscript; available in PMC: 2015 Mar 20.

Published in final edited form as: Mol Pharmacol. 2002 Nov;62(5):1249–1257. doi: 10.1124/mol.62.5.1249

Fig. 2 — P2Y1-R selective binding of [³H]MRS2279 in membranes prepared from Sf9 cells. Sf9 membranes prepared from wild-type cells or cells expressing the recombinant human P2Y1, P2Y2, or P2Y12 receptors (30 µg/assay, A) were incubated with 6 nM [³H]MRS2279 in the absence () or the presence () of 10 µM 2MeSADP. The specific binding of [³H]MRS2279 (defined with 10 µM 2MeSADP) was determined over the indicated range of membrane protein for each preparation (B).

Inline graphic — P2Y1-R selective binding of [³H]MRS2279 in membranes prepared from Sf9 cells. Sf9 membranes prepared from wild-type cells or cells expressing the recombinant human P2Y1, P2Y2, or P2Y12 receptors (30 µg/assay, A) were incubated with 6 nM [³H]MRS2279 in the absence () or the presence () of 10 µM 2MeSADP. The specific binding of [³H]MRS2279 (defined with 10 µM 2MeSADP) was determined over the indicated range of membrane protein for each preparation (B).