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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Pharmacol. 2002 Nov;62(5):1249–1257. doi: 10.1124/mol.62.5.1249

Fig. 3.

Fig. 3

Association rate kinetics of [3H]MRS2279 binding in P2Y1-Sf9 membranes. P2Y1-Sf9 membranes (10 µg/assay) were incubated for the indicated times at 4°C with 5.8 nM [3H]MRS2279 and with or without 10 µM 2MeSADP before rapid filtration through GF/A filters as described under Materials and Methods. The data shown are specific binding of the radioligand. Inset, pseudo–first-order rate plot.