Table 1.
Primers used for the amplification of DNA of Babesia spp., Hepatozoon spp., Anaplasma spp., Bartonella spp ., ‘ Candidatus Neoehrlichia mikurensis’, Ehrlichia canis , Rickettsia spp. and filaroid nematodes
| Specifity | Genetic marker | Sequences of primers (5’-3’) | Lenght of amplicons (bp) | Reference |
|---|---|---|---|---|
| Apicomplexa | 18S rRNA | BTH-1 F: CCT GAG AAA CGG CTA CCA CAT CT | 561 | [45] |
| BTH-1R: TTG CGA CCA TAC TCC CCC CA | ||||
| Babesia spp. | Nested PCR: | |||
| Theileria spp. | GF2: GTC TTG TAA TTG GAA TGA TGG | |||
| GR2: CCA AAG ACT TTG ATT TCT CTC | ||||
| Hepatozoon canis | 18S rRNA | H14Hepa18SFw: GAA ATA ACA ATA CAA GGC AGT TAA AAT GCT | 620 | present study |
| H14Hepa18SRv: GTG CTG AAG GAG TCG TTT ATA AAG A | ||||
| Anaplasmataceae | 16S rRNA | EHR16SD: GGT ACC YAC AGA AGA AGT CC | 345 | [46] |
| EHR16SR: TAG CAC TCA TCG TTT ACA GC | ||||
| Bartonella spp. | 16S-23S rRNA | bartg_for: GAT GAT GAT CCC AAG CCT TC | 134 - 315 | modified [47] |
| B1623_rev: AAC CAA CTG AGC TAC AAG CC | ||||
| Rickettssia spp. | 23S/5S rRNA | ITS-F: GAT AGG TCG GGT GTG GAA G | 342 - 533 | [48] |
| ITS-R: TCG GGA TGG GAT CGT GTG | ||||
| Filaroid nematodes | COI | H14FilaCOIFw: GCC TAT TTT GAT TGG TGG TTT TGG | 724 | present study |
| H14FilaCOIRv: AGC AAT AAT CAT AGT AGC AGC ACT AA |