Table 1.
Enzyme | Substrate |
kcat (min−1) |
KM (µM) |
kcat/KM (min−1 M−1) |
RCEa |
Kss (µM) |
b |
---|---|---|---|---|---|---|---|
mBChE | (−)-cocaine | 1.4 | 1.6 | 8.8 × 105 | 1 | 1300 | 3.13 |
mCocH | (−)-cocaine | 250 | 35 | 7.1 × 106 | 8.2 | 1500 | 0.88 |
hBChEb | (−)-cocaine | 4.1 | 4.5 | 9.1 × 105 | 1 | 216 | 1.80 |
hCocHc | (−)-cocaine | 5700 | 3.1 | 1.8 × 109 | 2020 | 137 | 0.40 |
mBChE | ACh | 38400 | 400 | 9.6× 107 | 1 | 15200 | 1.79 |
mCocH | ACh | 19000 | 210 | 9.0 × 107 | 0.94 | 25800 | 0.30 |
hBChEd | ACh | 61200 | 148 | 4.1 × 108 | 1 | 31600 | 2.58 |
hCocHe | ACh | 11900 | 37 | 3.2 × 108 | 0.78 | 30000 | 0.68 |
mBChE | BTC | 35600 | 72 | 4.9 × 108 | 1 | 5000 | 2.77 |
mCocH | BTC | 28200 | 99 | 2.8 × 108 | 0.58 | 254 | 0.19 |
hBChEf | BTC | 29500 | 17 | 1.7 × 109 | 1 | 3010 | 3.36 |
hCocHe | BTC | 28000 | 13 | 2.2 × 109 | 1.24 | 243 | 0.49 |
RCE refers to the relative catalytic efficiency (kcat/KM), i.e. the ratio of the kcat/KM value of a mutant (mCocH or hCocH) to that of the corresponding wild-type enzyme (mBChE or hBChE) against the same substrate.
The kcat and KM for hBChE against (−)-cocaine were reported in ref.([8]).
The kcat and KM for hCocH against (−)-cocaine were reported in ref.([16]).
The kcat and KM for hBChE against ACh were reported in ref.([50]).
The kcat and KM for hCocH against ACh and BTC were reported in ref.([51]).
The kcat and KM for hBChE against BTC were reported in ref.([20]).