FIG. 1.

Pretreatment with Dexra prevents DXR-induced DNA damage in marmoset ovarian cells. NCA summary data indicate pretreatment with Dexra prevented an acute DXR-induced increase in DNA DSBs in pooled ovarian cells in marmoset ovarian tissue at 4 h post-DXR treatment. For each individual experiment, the increases in DNA DSBs caused by both doses of DXR exceeded control values, while Dexra pretreatment was similar to control baseline. In vitro cultured marmoset ovarian tissue slices were pretreated with vehicle for 1 h followed by 50 or 500 nM DXR or with 20 μM Dexra for 1 h followed by 50 nM DXR treatment. Ovarian tissue slices were harvested at 4 h post-DXR treatment. DNA damage was quantified in pooled somatic ovarian cells as the Olive moment, summarized in a bar graph of data pooled from three experiments (n = 3 marmosets; each experimental sample was run in duplicate, >100 somatic cells per data collection point, *P < 0.05, one-way ANOVA).