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. 2015 Jan 21;92(3):73. doi: 10.1095/biolreprod.114.119495

FIG. 2.

FIG. 2

Dexra pretreatment attenuates DXR-induced H2AX phosphorylation in marmoset ovarian tissue protein lysates. Marmoset ovarian tissue culture slices were pretreated with vehicle control (Ctl) for 1 h followed by 50 or 500 nM DXR or with 20 μM Dexra 1 h followed by 50 nM DXR treatment. Cultured ovarian tissue slices from four marmosets were harvested at 4 h post-DXR treatment. A) Representative Western blots from one animal probed with antibodies against phospho-γH2AX and β-actin (loading control) as indicated. B and C) Summary bar graphs of γH2AX phosphorylation from two individual marmoset animals exhibiting increased γH2AX phosphorylation at 4 h after 50 nM DXR (n = 4). Dexra significantly decreased DXR-induced γH2AX phosphorylation in one animal (B) and diminished the levels of p-γH2AX phosphorylation below control in the second animal (C).