FIG. 3.

Dexra pretreatment decreases the number of follicles containing phospho-γH2AX foci-positive granulosa cells. Marmoset ovarian tissue culture slices were treated with vehicle control or DXR ± Dexra and fixed at 24 h post-DXR treatment. Ovarian sections were stained for p-γH2AX (green) and nuclei (blue, DAPI). A) Representative fluorescent images (A–H) demonstrate formation of p-γH2AX foci in marmoset ovarian sections for each treatment condition from the same animal (A and B = control, C and D = 50 nM DXR, E and F = Dexra + 50 nM DXR, G and H = 500 nM DXR). Bar = 500 μm for all the images. B) Summary bar graphs of p-γH2AX-positive antral follicles at 24 h after 50 and 500 nM DXR treatment ± Dexra. A growing follicle is considered positive if it contains at least four p-γH2AX-positive granulosa cells per follicle; *P < 0.05 and **P < 0.001, one-way ANOVA, Bonferroni means comparison (n = 3 animals).