FIG. 6.

Ratio of cleaved caspase-9 over total procaspase-9 levels in marmoset ovarian tissue tended to increase following DXR treatment, but not following DXR and Dexra pretreatment. Marmoset ovarian tissue culture slices were pretreated with vehicle control for 1 h followed by the addition of 50 or 500 nM DXR or with 20 μM Dexra 1 h prior to 50 nM DXR treatment. Ovaries were harvested 24 h post-DXR treatment (n = 3). A) Western blots probed with caspase-9 antibodies and β-actin as the loading control. B) Bar graph quantification of caspase-9 band density from ovarian tissue protein lysates. Lysates from ovarian tissue slices treated with DXR tended to exceed values for DXR with Dexra pretreatment or controls for ratios of cleaved caspase-9 over total procaspase-9 levels in marmoset ovarian tissue.