FIG. 7.

AKT1 phosphorylation in marmoset ovarian tissue tended to increase following DXR treatment, but not following DXR and Dexra pretreatment. Western blot analysis of pAKT1 and PTEN proteins in marmoset ovarian tissue culture treated with vehicle control, DXR, or DXR ± Dexra. A) Western blot from one marmoset at 24 h post-DXR treatment probed with anti-pAKT1 and β-actin antibodies as indicated. B) Summary bar graph of Western blot data quantified from four marmosets at 24 h post-DXR treatment. DXR treatment tended to enhance induction of AKT1 phosphorylation at the 24-h time point, and this trend was obscured by Dexra pretreatment (n = 4). C) Example of Western blot of lysates of ovarian tissue harvested 4 h post-DXR probed with anti-PTEN and β-actin antibodies as indicated. D) Summary bar graph demonstrates no DXR-induced change in PTEN protein expression levels, with or without Dexra pretreatment, at 4 h posttreatment.