Figure 4.

Compound 4hh dose-dependently increases viability in SH-SY5Y and H4 cells. SH-SY5Y or H4 cells were pretreated with 4hh for 2 h and then treated with 7.5 μM thapsigargin for an additional 18 h. Cell viability was assessed using the CellTiter 96 AQ_ueous One Solution Cell Proliferation Assay. Wells containing 100 μL of DMEM but no cells were used as background, whereas wells treated with DMSO but no thapsigargin were used as positive controls. The viability (% of control) = 100 × (well value – average of background)/(average of positive control – average of background). Experiments were repeated at least three times, and the data are represented as the average ± SEM.