. Author manuscript; available in PMC: 2015 Jun 25.

Published in final edited form as: Mol Cell Endocrinol. 2014 Apr 30;391(0):1–9. doi: 10.1016/j.mce.2014.04.011

Table 5.

Photoperiodic Regulation of Testicular mRNA Levels

A. cDNA Clones from ACP-DD-PCR Screen
cDNA	LD	SD-S	SD-R	Samples	F-statistic	p-value
Aqp11	0.53 ± 0.05	0.25 ± 0.05^**	0.45 ± 0.05^†	n = 6-7	F_2,17 = 8.64	0.003
At7l1	1.46 ± 0.29	0.61 ± 0.11^**	1.28 ± 0.23^††	n = 5	F_2,12 = 4.04	0.046
Coro1c	1.13 ± 0.10	1.11 ± 0.17	0.99 ± 0.13	n = 5	F_2,12 = 0.31	0.740
Dusp3	1.92 ± 0.18	0.71 ± 0.19^**	1.25 ± 0.10	n = 6-7	F_2,17 = 13.38	0.001
Jls1	N/D	N/D	N/D	n = 5	N/D	N/D
Niam	1.04 ± 0.07	0.66 ± 0.12^**	1.01 ± 0.10^†	n = 6-7	F_2,17 = 4.69	0.024
Und1	N/D	N/D	N/D	n = 5	N/D	N/D
Und2	N/D	N/D	N/D	n = 5	N/D	N/D
Zfp639	0.51 ± 0.05	0.51 ± 0.05	0.55 ± 0.08	n = 5	F_2,12 = 0.14	0.871

B. Reference cDNA Clones
cDNA	LD	SD-S	SD-R	Samples	F-statistic	p-value
Bax	1.42 ± 0.15	1.66 ± 0.09	1.15 ± 0.12^†	n = 5	F_2,12 = 4.34	0.038
Bcl2	0.82 ± 0.20	0.89 ± 0.07	0.61 ± 0.13^†	n = 5	F_2,12 = 1.03	0.386
Catsper1	1.26 ± 0.17	0.42 ± 0.15^*	1.58 ± 0.27^††	n = 6-7	F_2,17 = 9.33	0.002
Cox2	0.41 ± 0.12	0.67 ± 0.15	0.63 ± 0.08	n = 5	F_2,12 = 1.36	0.294
3βHsd	0.59 ± 0.06	0.24 ± 0.05^**	0.36 ± 0.05^*	n = 5	F_2,12 = 11.04	0.002
Hspa2	0.70 ± 0.09	0.44 ± 0.07	0.43 ± 0.14	n = 6-7	F_2,17 = 2.39	0.122
Pgk2	1.14 ± 0.13	0.43 ±0.16^**	0.99 ± 0.07^††	n = 6-7	F_2,17 = 8.54	0.003
Srepb2	0.61 ± 0.07	0.35 ± 0.06^*	0.64 ± 0.06^†	n = 6-7	F_2,17 = 6.29	0.009
Tnp2	0.52 ± 0.11	0.17 ± 0.10^*	0.50 ± 0.07^†	n = 6-7	F_2,17 = 4.19	0.033

Testis mRNA levels were assessed by RT-PCR for hamsters (n = 5-7) exposed to 16 weeks of long days (LD, 14L:10D), 16 weeks of short days during photosensitivity (SD-S, 10L:14D), or 22 weeks of SD during photorefractoriness (SD-R, 10L:14D). (A) cDNA clones screened by ACP-DD-PCR and normalized after Actb co-amplification. ANOVA for mRNA expression levels between groups (F-statistic) are shown. (B) Reference cDNA normalized after Actb co-amplification. Means (± SEM) are shown. For SD-S or SD-R vs LD

p < 0.050

^**

p < 0.010.

For SD-S vs SD-R comparisons

^†

p < 0.050

^††

p < 0.010. N/D, not determined. Degrees of freedom (associated with F-statistics) represent treatments between groups (i.e., 2) and residuals within groups (i.e., 12 or 17).