Figure 2.

Lv-mediated silencing of SNRPN in Daoy cells. (A) Determination of infection efficiency in Daoy cells. Representative images of Daoy cells after five days of Lv infection are shown (scale bar, 50 μm). (B and C) Expression analysis of SNRPN mRNA and protein levels in Daoy cells following one of three treatments (Con, Lv-shCon or Lv-shSNRPN) by (B) RT-qPCR and (C) western blotting. β-actin gene and GAPDH protein served as internal controls for RT-qPCR and western blotting, respectively. (^**P<0.01). Lv, lentivirus; SNRPN, small nuclear ribonucleoprotein-associated polypeptide N; RT-qPCR, reverse transcription-polymerase chain reaction; Con, control, sh, short hairpin; GFP, green fluorescence protein.