Figure 4.

Effect of Gö6983 on opioid-induced ERK activation in SH-SY5Y cells. Cells were pretreated with 1 μM Gö6983 or vehicle (dimethyl sulfoxide) for 30 min and were then stimulated with (A) 1 μM DAMGO or (B) 1 μM morphine for 5 min. Data are presented as the mean ± standard error of the mean from three independent experiments. Two-way analysis of variance was used followed by the Bonferroni post hoc test. ^***P<0.001, compared with each control. PEBP, phosphatidylethanolamine-binding protein; DAMGO, (D-Ala², MePhe⁴, Gly⁵-ol) enkephalin; ERK, extracellular signal-regulated kinase; CHO cells, Chinese hamster ovary cells.