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. 2015 Mar 20;10(3):e0120646. doi: 10.1371/journal.pone.0120646

Fig 5. Enhanced drought sensitivity of transgenic N. benthamiana overexpressing GhWRKY68.

Fig 5

(A) Seed germination on 1/2 MS medium with 0, 100, or 200 mM mannitol. (B) The germination rates of the WT and OE lines in 0 or 200 mM mannitol treatment conditions are presented. Germination was scored daily. (C) Representative phenotypes of 8-week-old WT and OE plants grown in soil before or after 10 days of drought. (D) Survival rates of WT and OE plants under drought conditions. (E) Representative phenotypes of the detached leaves of WT and OE plants under dehydration conditions. (F) Relative water loss rates from detached leaves of WT and OE plants were measured after dehydration at the indicated times. (G-H) Stomatal changes observed with a microscope before and after drought treatment. The stomatal length: width ratio is displayed. BD, before drought treatment; RW, re-watering. The data presented are the means ± SE of three independent experiments (n = 3). Different letters above the columns in (D) and (H) indicate significant differences (P < 0.0001) according to Duncan’s multiple range test performed using SAS version 9.1 software.