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. 2015 Mar 20;10(3):e0120646. doi: 10.1371/journal.pone.0120646

Fig 7. Overexpression of GhWRKY68 negatively regulates ABA signalling in transgenic plants.

Fig 7

(A) Seed germination of WT and OE lines on 1/2 MS medium containing different ABA concentrations (0, 2 or 5 μM). (B) Germination rates of the WT and OE lines under 0 or 5 μM ABA treatment conditions. Germination was scored daily. (C) Root elongation of WT and OE plants after treatment with different ABA concentrations (0, 2 or 5 μM). (D) Statistical analysis of the root length. (E) Micrographs showing the stomatal closure of transgenic and WT plants with or without 20 μM ABA treatment. (F) Stomatal closure response to ABA treatment. The data represent the means ± SE of 40 stomata from three independent experiments. (G) ABA contents of WT and OE plants before and after treatment. For drought treatment, 8-week-old OE and WT plants were grown in soil without water for 10 days. For salt treatment, WT and OE plants were irrigated with salt water (200 mM) for 1 month. The values represent the means ± SE of three independent experiments. The different letters above the columns indicate significant differences (P < 0.05) according to Duncan’s multiple range test.